The newly upgraded Bruker AVANCE500 spectrometer is a narrow bore, high resolution, nmr spectrometer that is being used for both solution and solid-state NMR spectroscopy. It is a four channel spectrometer that operates at 11.7T (1H frequency of 500MHz). It is equipped with various double resonance Broardband Probeheads for both solution (10mm BBO 1H/X Double Resonance Probe) and solids (2.5mm, 4mm, and 5mm MAS 1H/X Probes), Triple-Resonance/Triple-Gradients Probe for biological solution NMR, Triple/Resonance Transmission Line Probe (under building) for solids, and 4mm hr-MAS 1H/13C z-gradient Probe for macromolecules and oriented media in solution. See the Table for more probe details.

The AVANCE500 spectrometer has comprehensive modern NMR capabilities and covers a wide range of NMR experiments in both solution- and solid-state, as shown below.

• Solution NMR:
• Everything which can be done on AVANCE200, and the same 1D NMR and 2D NMR operation procedures can be followed for solution NMR.
• Features:
• 4-Channels: 1H, X, Y, and Z
• Variety of probes (see Probe List below)
• Digital Filtering and Oversampling
• Deuterium Decoupling (to be installed) and Observe
• 0.05¡ phase stability, 0.001 Hz frequency resolution, ±0.1 °C temperature control, and linear power amplifiers
• 3-axis Gradients:
• DOSY for diffussion measurement
• Gradient Shimming
• Gradient Solvent Suprresion (WET,MA,WATERGATE)
• etc.
• Macromolecular Study:
  Proteins, DNAs, RNAs, Protein-DNA(RNA) complexes, Lipid Bilayers, etc.
• 3D Structure and Dynamics:
  NOESY, HSQC-NOESY, TOCSY-NOESY, HNCA, HNCACB, HNCO, TROSY, HCCH-TOCSY, and all other experiments for biological NMR

• Solid-state NMR:
  • Super High Sample Spinning Rate up to 35 kHz
  • High Power Amplifiers: 1kW for both 1H and BB channel
  • Fast Digitizer: 12 bit FADC with 10 MHz sampling rate
  • Sample Changer (without removing probe from magnet)
  • Variable Temperature (see Table for Probes below)
  • Samples: Polymer (Plastic), Biopolymer (Protein, Lipid), Catalyst (Zeolite), etc.
  • Application:
  • Activation Energy, Chemical Shift, CSA, Quadrupolar Parameters(CQ, h)..
  • Correlation, Exchange, Motion, Relaxation, ...
  • Spectral Editting, Connectivity, Distance, Geometry, Orientation, ...
  • Experiments for I=1/2 Nuclei (see NMR Periodic Table below):
    BD, CPMAS, CRAMPS (1H Observe), 19F-Observe/Decoupling, (CPEDIT, SS-APT), (MAS-J-HMQC, INADEQUATE, HETCOR, J-HETCOR), (C7, DRAW, MELODRAMA, R2,REDOR, RFDR, TEDOR), etc.
  • Experiments for I>1/2 Nuclei (see NMR Periodic Table below):
    CP-MQMAS, MAS, MQMAS, MQMAS-HETCOR, MQ-REDOR, QUADECHO, REAPDOR, RIACT, TRAPDOR, etc.

  For more details of individual nucleis, please see:

  • NMR Frequecy
  • NMR Periodic Table
  • Properties of Nuclei